human escc cell line te13  (ATCC)

Journal: Oncology Letters

Article Title: The role of the retinoblastoma protein-interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells

doi: 10.3892/ol.2013.1608

Figure Lengend Snippet: Western blot analysis of RIZ1 protein expression in TE13 cells transfected with pcDNA3.1(+)/RIZ1. β-actin was used as a loading control. Untransfected TE13 cells were used as a control. Significantly higher RIZ1 protein expression (P<0.01) was observed in the pcDNA3.1(+)/RIZ1-transfected cells, compared with the empty vector and untransfected controls. RIZ1, retinoblastoma protein-interacting zinc finger gene 1.

Article Snippet: The human ESCC TE13 cell line was purchased from American Type Culture Collection (Rockville, MD, USA) and cultured in RPMI-1640 containing 4.76 g HEPES, 2.0 g NaCO 3 , 10.4 g RPMI-1640 and 1,000 ml ddH 2 O (Gibco, Carlsbad, CA, USA), supplemented with 10% fetal bovine serum (Gibco), 1X L-glutamine (2 mM), 100 U/ml penicillin and 100 μg/ml streptomycin.

Techniques: Western Blot, Expressing, Transfection, Control, Plasmid Preparation

Journal: Oncology Letters

Article Title: The role of the retinoblastoma protein-interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells

doi: 10.3892/ol.2013.1608

Figure Lengend Snippet: qPCR analysis of RIZ1 mRNA expression in TE13 cells transfected with pcDNA3.1(+)/RIZ1. RIZ1 mRNA expression was normalized to that of GAPDH. The difference was statistically insignificant between the empty vector and untransfected controls groups (P>0.05), whereas the deviation between the comparison groups and the pcDNA3.1(+)/RIZ1-transfected group was statistically significant (P<0.01). qPCR, quantitative polymerase chain reaction; RIZ1 , retinoblastoma protein-interacting zinc finger gene 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: The human ESCC TE13 cell line was purchased from American Type Culture Collection (Rockville, MD, USA) and cultured in RPMI-1640 containing 4.76 g HEPES, 2.0 g NaCO 3 , 10.4 g RPMI-1640 and 1,000 ml ddH 2 O (Gibco, Carlsbad, CA, USA), supplemented with 10% fetal bovine serum (Gibco), 1X L-glutamine (2 mM), 100 U/ml penicillin and 100 μg/ml streptomycin.

Techniques: Expressing, Transfection, Plasmid Preparation, Comparison, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: The role of the retinoblastoma protein-interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells

doi: 10.3892/ol.2013.1608

Figure Lengend Snippet: Flow cytometric analysis of the rate of apoptosis in the TE13 cells that were transfected with pcDNA3.1(+)/RIZ1. (A) Representative flow cytometric plots. (B) The rate of apoptosis was significantly higher in the pcDNA3.1(+)/RIZ1-transfected cells (P<0.01) than in the TE13 cells that were transfected with the empty pcDNA3.1(+) vector or the untransfected cells. RIZ1, retinoblastoma protein-interacting zinc finger gene 1; PI, propidium iodide; PE, phycoerythrein; FITC, fluorescein isothiocyanate.

Article Snippet: The human ESCC TE13 cell line was purchased from American Type Culture Collection (Rockville, MD, USA) and cultured in RPMI-1640 containing 4.76 g HEPES, 2.0 g NaCO 3 , 10.4 g RPMI-1640 and 1,000 ml ddH 2 O (Gibco, Carlsbad, CA, USA), supplemented with 10% fetal bovine serum (Gibco), 1X L-glutamine (2 mM), 100 U/ml penicillin and 100 μg/ml streptomycin.

Techniques: Transfection, Plasmid Preparation

Journal: Oncology Letters

Article Title: The role of the retinoblastoma protein-interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells

doi: 10.3892/ol.2013.1608

Figure Lengend Snippet: qPCR analysis of RIZ1 mRNA expression in the TE13 cells that were treated with 5-aza-CdR. RIZ1 mRNA level was expressed relative to that of GAPDH and calculated as 2 −averageΔΔCT × 100%. RIZ1 mRNA expression increased the longer the cells were treated with the drug (P<0.05). qPCR, quantitative polymerase chain reaction; RIZ1 , retinoblastoma protein-interacting zinc finger gene 1; 5-aza-CdR, 5-aza-2′-deoxycytidine; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: The human ESCC TE13 cell line was purchased from American Type Culture Collection (Rockville, MD, USA) and cultured in RPMI-1640 containing 4.76 g HEPES, 2.0 g NaCO 3 , 10.4 g RPMI-1640 and 1,000 ml ddH 2 O (Gibco, Carlsbad, CA, USA), supplemented with 10% fetal bovine serum (Gibco), 1X L-glutamine (2 mM), 100 U/ml penicillin and 100 μg/ml streptomycin.

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: The role of the retinoblastoma protein-interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells

doi: 10.3892/ol.2013.1608

Figure Lengend Snippet: Western blotting of RIZ1 protein expression in the TE13 cells that were treated with 5-aza-CdR. (A) TE13 cells were treated with 5-aza-CdR for 24, 48 or 72 h and subjected to western blotting. (B) Quantification of RIZ1 protein expression. 5-aza-CdR significantly increased RIZ1 protein expression compared with that of the control cells in 48 or 72 h (P<0.01) exclusive of the 24 h group. Treatment with 5-aza-CdR for 48 or 72 h significantly increased RIZ1 protein expression compared with that in the 24 h group (P<0.01). RIZ1, retinoblastoma protein-interacting zinc finger gene 1; 5-aza-CdR, 5-aza-2′-deoxycytidine.

Article Snippet: The human ESCC TE13 cell line was purchased from American Type Culture Collection (Rockville, MD, USA) and cultured in RPMI-1640 containing 4.76 g HEPES, 2.0 g NaCO 3 , 10.4 g RPMI-1640 and 1,000 ml ddH 2 O (Gibco, Carlsbad, CA, USA), supplemented with 10% fetal bovine serum (Gibco), 1X L-glutamine (2 mM), 100 U/ml penicillin and 100 μg/ml streptomycin.

Techniques: Western Blot, Expressing, Control

Journal: Oncology Letters

Article Title: The role of the retinoblastoma protein-interacting zinc finger gene 1 tumor suppressor gene in human esophageal squamous cell carcinoma cells

doi: 10.3892/ol.2013.1608

Figure Lengend Snippet: Flow cytometric analysis of the rate of apoptosis in the TE13 cells that were treated with 5-aza-CdR. (A) Representative flow cytometric plots. (B) Quantification of the apoptotic index. The rate of apoptosis increased with the time of exposure to 5-aza-CdR (P<0.01). 5-aza-CdR, 5-aza-2′-deoxycytidine; PI, propidium iodide; PE, phycoerythrein; FITC, fluorescein isothiocyanate.

Article Snippet: The human ESCC TE13 cell line was purchased from American Type Culture Collection (Rockville, MD, USA) and cultured in RPMI-1640 containing 4.76 g HEPES, 2.0 g NaCO 3 , 10.4 g RPMI-1640 and 1,000 ml ddH 2 O (Gibco, Carlsbad, CA, USA), supplemented with 10% fetal bovine serum (Gibco), 1X L-glutamine (2 mM), 100 U/ml penicillin and 100 μg/ml streptomycin.

Techniques: